CultivariumFungal DNA methylation

Fungal DNA methylation

We use Nanopore sequencing to identify DNA methylations in fungal genomes of these types: 5mC, 5hmC, and 6mA.

Each row reports methylated sites per modification class with both raw site counts and the fraction of target bases called methylated (cytosine modifications reported per C, adenine modifications per A). High-confidence sites are those with ≥5× coverage and >90% reads modified.

Methods note: basecalling with Dorado's 5mC_5hmC model (single call per cytosine distinguishing 5mC, 5hmC, and unmodified C) plus a separate 6mA model on adenines. Call assignment uses the standard modkit codes (m = 5mC / ChEBI 27551, h = 5hmC / ChEBI 76792, a = 6mA).

Species
Genome
Methylated sites — raw count and % of target bases
0%0.181%0.362%0.543%0.724%
Filter: ≥5× coverage, >90% reads modified (modkit 90th-percentile threshold BEDs).