Fungal DNA methylation
We use Nanopore sequencing to identify DNA methylations in fungal genomes of these types: 5mC, 5hmC, and 6mA.
Each row reports methylated sites per modification class with both raw site counts and the fraction of target bases called methylated (cytosine modifications reported per C, adenine modifications per A). High-confidence sites are those with ≥5× coverage and >90% reads modified.
Methods note: basecalling with Dorado's 5mC_5hmC model (single call per cytosine distinguishing 5mC, 5hmC, and unmodified C) plus a separate 6mA model on adenines. Call assignment uses the standard modkit codes (m = 5mC / ChEBI 27551, h = 5hmC / ChEBI 76792, a = 6mA).
Species
Genome
Methylated sites — raw count and % of target bases
0%0.181%0.362%0.543%0.724%
Coprinopsis cinerea
CCGCF_000182895.1
13,645 annotated genes
Size36.2 Mb
Contigs68
Cytosines18,689,911
Adenines17,502,645
5mC
128,9020.690% of C
5hmC
380.00020% of C
6mA
42.3e-5% of A
Rhizopus arrhizus
RAGCA_011764125.1
15,982 annotated genes
Size43.9 Mb
Contigs34,282
Cytosines16,687,881
Adenines27,232,632
5mC
610.00037% of C
5hmC
00% of C
6mA
60,0460.220% of A
Emergomyces africanus
EAGCA_001660665.1
8,858 annotated genes
Size29.7 Mb
Contigs4,444
Cytosines12,908,342
Adenines16,799,182
5mC
2630.0020% of C
5hmC
00% of C
6mA
00% of A
Candidozyma auris
CAGCA_007168705.1
5,445 annotated genes
Size12.1 Mb
Contigs12
Cytosines5,477,331
Adenines6,648,055
5mC
00% of C
5hmC
00% of C
6mA
11.5e-5% of A