Fungal DNA methylation
We use Nanopore sequencing to identify DNA methylations in fungal genomes of these types: 5mC, 5hmC, and 6mA. Each card reports methylated sites per modification class with raw counts and the fraction of target bases methylated; high-confidence sites have ≥5× coverage and >90% reads modified.
Methods note: basecalling with Dorado's 5mC_5hmC model (single call per cytosine distinguishing 5mC, 5hmC, and unmodified C) plus a separate 6mA model on adenines. Call assignment uses the standard modkit codes (m = 5mC / ChEBI 27551, h = 5hmC / ChEBI 76792, a = 6mA).
4 species
Coprinopsis cinerea
CCGCF_000182895.1
13,645 annotated genes
Size36.2 Mb
Contigs68
Cytosines18,689,911
Adenines17,502,645
5mC
128,9020.690% of C
5hmC
380.00020% of C
6mA
42.3e-5% of A
Rhizopus arrhizus
RAGCA_011764125.1
15,982 annotated genes
Size43.9 Mb
Contigs34,282
Cytosines16,687,881
Adenines27,232,632
5mC
610.00037% of C
5hmC
00% of C
6mA
60,0460.220% of A
Emergomyces africanus
EAGCA_001660665.1
8,858 annotated genes
Size29.7 Mb
Contigs4,444
Cytosines12,908,342
Adenines16,799,182
5mC
2630.0020% of C
5hmC
00% of C
6mA
00% of A
Candidozyma auris
CAGCA_007168705.1
5,445 annotated genes
Size12.1 Mb
Contigs12
Cytosines5,477,331
Adenines6,648,055
5mC
00% of C
5hmC
00% of C
6mA
11.5e-5% of A