CultivariumFungal electroporation
← Electroporation

Candidozyma auris

Ascomycota

Positive Results1

Waveform:Exponential
Result 1

Electroporation

Waveform
Exponential
Voltage
2 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 M Sorbitol

Media

Initial Growth
YEPD
Recovery
YEPD
Selection
YEPD

Culture Preparation

Morphology
Yeast
Antibiotic
Nourseothricin 50 µg/mL

Other Results17

AssessmentWaveformVoltage (kV)Duration (µs)Cap. (µF)Res. (Ω)Gap (µm)BufferMorph. FormPretreatmentFragmentationMelanizationInitial GrowthRecoverySelectionAntibiotic
NegativeExponential1.5—2560020001 M SucroseYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential2—2560020001 M SucroseYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential2.5—2560020001 M SucroseYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential1.5—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential2—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential2.5—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential1.5—2560020001 M SorbitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeExponential2.5—2560020001 M SorbitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare1.5600——20001 M SucroseYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare2600——20001 M SucroseYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare2.5600——20001 M SucroseYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare1.5600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare2600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare2.5600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare1.5600——20001 M SorbitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare2600——20001 M SorbitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL
NegativeSquare2.5600——20001 M SorbitolYeast———YEPDYEPDYEPDNourseothricin 50 µg/mL