CultivariumFungal electroporation
← Electroporation

Cryptococcus gattii

CVM600 · ATCC 14248

Basidiomycota

Other Results36

AssessmentWaveformVoltage (kV)Duration (µs)Cap. (µF)Res. (Ω)Gap (µm)BufferMorph. FormPretreatmentFragmentationMelanizationInitial GrowthRecoverySelectionAntibiotic
NegativeExponential1.5—2560020001 M SucroseYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential2—2560020001 M SucroseYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential2.5—2560020001 M SucroseYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential1.5—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential2—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential2.5—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential1.5—2560020001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential2—2560020001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential2.5—2560020001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential1.5—2560020001 M SucroseYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential2—2560020001 M SucroseYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential2.5—2560020001 M SucroseYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential1.5—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential2—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential2.5—2560020001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeExponential1.5—2560020001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential2—2560020001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeExponential2.5—2560020001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare2600——20001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare2.5600——20001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare1.5600——20001 M SucroseYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare2600——20001 M SucroseYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare2.5600——20001 M SucroseYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare1.5600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare2600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare2.5600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL
NegativeSquare1.5600——20001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare2600——20001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare2.5600——20001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare1.5600——20001 M SucroseYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare2600——20001 M SucroseYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare2.5600——20001 M SucroseYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare1.5600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare2600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare2.5600——20001 mM HEPES + 50 mM mannitolYeast———YEPDYEPDYEPDHygromycin 450 µg/mL
NegativeSquare1.5600——20001 M SorbitolYeast———YEPDYEPDYEPDHygromycin 400 µg/mL