CultivariumFungal electroporation
← Electroporation

Trichoderma reesei

CVM288 · NRRL 3652

Ascomycota

Other Results18

AssessmentWaveformVoltage (kV)Duration (µs)Cap. (µF)Res. (Ω)Gap (µm)BufferMorph. FormPretreatmentFragmentationMelanizationInitial GrowthRecoverySelectionAntibiotic
NegativeExponential1.5—2560020001 M SorbitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential2—2560020001 M SorbitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential2.5—2560020001 M SorbitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential1.5—2560020001 M SucroseSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential2—2560020001 M SucroseSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential2.5—2560020001 M SucroseSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential1.5—2560020001 mM HEPES + 50 mM mannitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential2—2560020001 mM HEPES + 50 mM mannitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeExponential2.5—2560020001 mM HEPES + 50 mM mannitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare1.5600——20001 M SorbitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare2600——20001 M SorbitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare2.5600——20001 M SorbitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare1.5600——20001 M SucroseSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare2600——20001 M SucroseSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare2.5600——20001 M SucroseSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare1.5600——20001 mM HEPES + 50 mM mannitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare2600——20001 mM HEPES + 50 mM mannitolSpores———PDBPDBPDBHygromycin 100 µg/mL
NegativeSquare2.5600——20001 mM HEPES + 50 mM mannitolSpores———PDBPDBPDBHygromycin 100 µg/mL