CultivariumFungal electroporation
← Electroporation

Neurospora crassa

Ascomycota

Positive Results13

Waveform:Exponential
Result 1

Electroporation

Waveform
Exponential
Voltage
1.5 kV
Capacitance
25 µF
Resistance
800 Ω
Gap Width
2000 µm
Buffer
1 M Sorbitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 2

Electroporation

Waveform
Exponential
Voltage
2 kV
Capacitance
25 µF
Resistance
800 Ω
Gap Width
2000 µm
Buffer
1 M Sorbitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 3

Electroporation

Waveform
Exponential
Voltage
1.5 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 M Sorbitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 4

Electroporation

Waveform
Exponential
Voltage
2 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 M Sorbitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 5

Electroporation

Waveform
Exponential
Voltage
1.5 kV
Capacitance
25 µF
Resistance
800 Ω
Gap Width
2000 µm
Buffer
1 M Sucrose

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 6

Electroporation

Waveform
Exponential
Voltage
2 kV
Capacitance
25 µF
Resistance
800 Ω
Gap Width
2000 µm
Buffer
1 M Sucrose

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 7

Electroporation

Waveform
Exponential
Voltage
2.5 kV
Capacitance
25 µF
Resistance
800 Ω
Gap Width
2000 µm
Buffer
1 M Sucrose

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 8

Electroporation

Waveform
Exponential
Voltage
1.5 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 M Sucrose

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 9

Electroporation

Waveform
Exponential
Voltage
2 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 M Sucrose

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 10

Electroporation

Waveform
Exponential
Voltage
2.5 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 M Sucrose

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 11

Electroporation

Waveform
Exponential
Voltage
1.5 kV
Capacitance
25 µF
Resistance
800 Ω
Gap Width
2000 µm
Buffer
1 mM HEPES + 50 mM mannitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 12

Electroporation

Waveform
Exponential
Voltage
1.5 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 mM HEPES + 50 mM mannitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL
Result 13

Electroporation

Waveform
Exponential
Voltage
2 kV
Capacitance
25 µF
Resistance
600 Ω
Gap Width
2000 µm
Buffer
1 mM HEPES + 50 mM mannitol

Media

Initial Growth
VMM
Recovery
NRS
Selection
NRS

Culture Preparation

Morphology
Spores
Antibiotic
Hygromycin 400 µg/mL

Other Results42

AssessmentWaveformVoltage (kV)Duration (µs)Cap. (µF)Res. (Ω)Gap (µm)BufferMorph. FormPretreatmentFragmentationMelanizationInitial GrowthRecoverySelectionAntibiotic
UncertainExponential1.52580020001 M SorbitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeExponential22580020001 M SorbitolSporesVMMNRSNRSHygromycin 800 µg/mL
UncertainExponential2.52580020001 M SorbitolSporesVMMNRSNRSHygromycin 800 µg/mL
UncertainExponential1.52580020001 M SucroseSporesVMMNRSNRSHygromycin 800 µg/mL
UncertainExponential22580020001 M SucroseSporesVMMNRSNRSHygromycin 800 µg/mL
UncertainExponential2.52580020001 M SucroseSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeExponential1.52580020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeExponential22580020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 800 µg/mL
UncertainExponential2.52580020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeExponential2.52580020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeExponential2.52560020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeExponential22580020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeExponential2.52580020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeExponential2.52560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeExponential1.52560020001 M SorbitolMyceliavortex + beadYEPDNRSNRSHygromycin 400 µg/mL
NegativeSquare1.560020001 M SorbitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare260020001 M SorbitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare2.560020001 M SorbitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare1.560020001 M SucroseSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare260020001 M SucroseSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare2.560020001 M SucroseSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare1.560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare260020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare2.560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 800 µg/mL
NegativeSquare1.560020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare260020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
UncertainSquare2.560020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare1.560020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare260020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare2.560020001 M SorbitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare1.560020001 M SucroseSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare260020001 M SucroseSporesVMMNRSNRSHygromycin 400 µg/mL
UncertainSquare2.560020001 M SucroseSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare1.560020001 M SucroseSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare260020001 M SucroseSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare2.560020001 M SucroseSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare1.560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare260020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
UncertainSquare2.560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare1.560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare260020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL
NegativeSquare2.560020001 mM HEPES + 50 mM mannitolSporesVMMNRSNRSHygromycin 400 µg/mL