Positive Results3
Waveforms:ExponentialSquare
Result 1
Electroporation
- Waveform
- Exponential
- Voltage
- 2.5 kV
- Capacitance
- 25 µF
- Resistance
- 600 Ω
- Gap Width
- 2000 µm
- Buffer
- 1 M Sorbitol
Media
- Initial Growth
- PDB
- Recovery
- PDB
- Selection
- PDB
Culture Preparation
- Morphology
- Mycelia
- Pretreatment
- 50 mM DTT
- Fragmentation
- vortex + bead
- Antibiotic
- Hygromycin 200 µg/mL
Result 2
Electroporation
- Waveform
- Square
- Voltage
- 2 kV
- Duration
- 600 µs
- Gap Width
- 2000 µm
- Buffer
- 1 M Sorbitol
Media
- Initial Growth
- PDB
- Recovery
- PDB
- Selection
- PDB
Culture Preparation
- Morphology
- Mycelia
- Pretreatment
- 50 mM DTT
- Fragmentation
- vortex + bead
- Antibiotic
- Hygromycin 200 µg/mL
Result 3
Electroporation
- Waveform
- Square
- Voltage
- 2 kV
- Duration
- 600 µs
- Gap Width
- 2000 µm
- Buffer
- 1 M Sucrose
Media
- Initial Growth
- PDB
- Recovery
- PDB
- Selection
- PDB
Culture Preparation
- Morphology
- Mycelia
- Pretreatment
- 50 mM DTT
- Fragmentation
- vortex + bead
- Antibiotic
- Hygromycin 200 µg/mL
Other Results16
| Assessment | Waveform | Voltage (kV) | Duration (µs) | Cap. (µF) | Res. (Ω) | Gap (µm) | Buffer | Morph. Form | Pretreatment | Fragmentation | Melanization | Initial Growth | Recovery | Selection | Antibiotic |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Uncertain | Exponential | 1.5 | — | 25 | 600 | 2000 | 1 M Sorbitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 2 | — | 25 | 600 | 2000 | 1 M Sorbitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 1.5 | — | 25 | 600 | 2000 | 1 M Sucrose | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 2 | — | 25 | 600 | 2000 | 1 M Sucrose | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 2.5 | — | 25 | 600 | 2000 | 1 M Sucrose | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 1.5 | — | 25 | 600 | 2000 | 1 mM HEPES + 50 mM mannitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 2 | — | 25 | 600 | 2000 | 1 mM HEPES + 50 mM mannitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Uncertain | Exponential | 2.5 | — | 25 | 600 | 2000 | 1 mM HEPES + 50 mM mannitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Exponential | 1.5 | — | 25 | 600 | 2000 | 1 M Sorbitol | Mycelia | — | vortex + bead | — | PDB | PDB | PDB | Hygromycin 100 µg/mL |
| Negative | Square | 1.5 | 600 | — | — | 2000 | 1 M Sorbitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Square | 2.5 | 600 | — | — | 2000 | 1 M Sorbitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Square | 1.5 | 600 | — | — | 2000 | 1 M Sucrose | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Square | 2.5 | 600 | — | — | 2000 | 1 M Sucrose | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Square | 1.5 | 600 | — | — | 2000 | 1 mM HEPES + 50 mM mannitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Square | 2 | 600 | — | — | 2000 | 1 mM HEPES + 50 mM mannitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |
| Negative | Square | 2.5 | 600 | — | — | 2000 | 1 mM HEPES + 50 mM mannitol | Mycelia | 50 mM DTT | vortex + bead | — | PDB | PDB | PDB | Hygromycin 200 µg/mL |